If they are in range of eachother I would probably use 1 and 3 to keep them from possibly interfering with eachother. It is also being assumed that you are talking about DMX here since this does not work with PixelNet. Unless all of your controllers are connecting wireless, you may want to arrange your channels to keep all of the wireless ones in the same universe and then you would only need 1 transmitter. Also remember that in DMX, each universe requires its own separate wiring system for the signal so at a minimum, try and keep each universe in its own area in the display to help cut down on cabling.