Ok, 2 things. First did you set the 2nd NIC to address 10.0.0.1? It doesn't look like it. I don't see it in your route table and it should be there.
Second, it looks like you need to run CMD.exe as Administrator to not get the elevation error in windows 7. In the search window for windows type CMD.exe. The result should come up under programs. Then you can right click it an select Run as Administrator. Then you should be able to get the ROUTE command to work.